The microscope is an optical instrument that has the remarkable ability to magnify tiny objects.

Since its invention, the microscope has played an indispensable role in scientific research, medical diagnosis, and industrial production.

The advent of the microscope has not only significantly expanded human perception but also brought about revolutionary changes in the field of science. It has enabled us to explore and understand the microscopic world, invisible to the eye.

The history of the microscope can be traced back to the 17th century. Dutch opticians Hans Jansen and his son, Zacharias Jansen, are often credited with being among the first to invent microscopes. However, Antonie van Leeuwenhoek truly popularized the microscope and applied it to scientific research.

Through the single-lens microscope he created, Leeuwenhoek was the first to observe and describe tiny structures such as bacteria, red blood cells, and sperm. His work unveiled the mysteries of the microbial world, marking a significant milestone in the history of science.

The basic principle of a microscope is to use a lens or a group of lenses to magnify objects. By altering the light path, these lenses produce a magnified virtual image of tiny objects. The earliest microscopes were simple single-lens microscopes, which could only magnify objects to a limited degree.

However, with the development of optical technology, compound microscopes gradually replaced single-lens microscopes and became mainstream. This type of microscope uses two sets of lenses—the objective lens and the eyepiece—working together to achieve higher magnification and clearer imaging.

The optical microscope, the earliest and most commonly used type of microscope, employs visible light and a set of optical lenses to magnify the image of a sample.

The resolution of an optical microscope typically reaches about 200 nanometers, which is sufficient for studying larger microscopic structures such as cells and microbial morphology. However, as scientific research advanced, the demand for higher resolution grew, leading to the invention of the electron microscope.

Electron microscopes offer a resolution far beyond that of optical microscopes by using electron beams instead of light beams to form images. Electron microscopes are broadly categorized into two types: transmission electron microscopes (TEM) and scanning electron microscopes (SEM).

Transmission electron microscopes allow electron beams to penetrate samples, providing detailed images of their internal structures with a resolution of about 0.1 nanometers.

Scanning electron microscopes, on the other hand, generate high-resolution three-dimensional surface images by detecting electrons reflected or emitted from the sample's surface.

These two types of electron microscopes have been widely used in fields such as materials science, biology, and nanotechnology, providing unprecedented observational capabilities for scientific research.

In addition to optical and electron microscopes, scanning probe microscopes (SPMs) developed in recent years have also garnered widespread attention in the scientific community.

Scanning probe microscopes use probes to scan the surface of a sample and measure the interaction force between the sample and the probe, thereby obtaining height information on the sample surface.

Scanning tunneling microscopes (STM) and atomic force microscopes (AFM) are representative technologies within this category that can achieve atomic-level resolution. These tools are so precise that they can even be used to manipulate individual atoms.

With the advancement of these microscope technologies, scientists can now not only observe the arrangement of atoms and molecules but also design and manipulate materials at the nanoscale. This capability has laid the foundation for the rapid development of nanoscience and nanotechnology.

The advancement of microscope technology has not only fueled the development of scientific research but has also played an irreplaceable role in medical diagnosis.

For example, optical microscopes are widely used to examine pathological tissue sections, aiding doctors in diagnosing diseases such as cancer and infections.

Fluorescence microscopes, which allow specific cells or molecules to be tagged with fluorescent markers, enable clearer observation of particular structures or processes in biological samples.

This is of great significance for studying the pathogenesis of diseases and developing new drugs. Additionally, electron microscopes are instrumental in observing the structure of viruses, a capability that is crucial for vaccine development and virology research.

As a powerful tool for scientific research, microscopes have vastly expanded human knowledge of the microscopic world. As technology continues to advance, microscopes will persist in pushing the boundaries of science, leading us into a more microscopic and intricate world.